NGS sample preparation reagents: KAPA library prep kits for DNA ...

Library construction is the first and a critical step in the next generation sequencing (NGS) workflow. DNA or RNA samples are first fragmented to the appropriate size and then sequencing platform-specific adapters are added. In most cases, libraries have to be amplified before the next step in the process (target enrichment or sequencing). Each step can potentially introduce errors or biases that could significantly impact library quality and ultimately affect the quality and reliability of sequencing results. It is therefore imperative to use the highest quality reagents and optimized protocols to obtain the best sequencing libraries, lowest bias, and the highest and most uniform coverage.

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Roche Sequencing Solutions offers a range of library preparation kits that deliver the highest quality sequencing libraries suitable for use on Illumina sequencing platforms. Kits contain high-quality enzymes selected through our Directed Evolution Technology, and formulated in convenient, easy-to-use master mixes. 

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The Quick-ITS Plus NGS Library Prep Kit (UDI) is the fastest and simplest NGS library prep targeting the ITS region for high-throughput sequencing, now with unique dual indexing for improved barcode resolution. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure® bead-based clean-ups. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.

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